QQ.1. Explain the principle and application of recombinant DNA technology.
- Recombinant DNA technology combines genetic material from different sources to create novel DNA sequences.
- Restriction enzymes cut DNA at specific sites; DNA ligase joins DNA fragments.
- Recombinant DNA is introduced into host cells (e.g., bacteria) for replication and gene expression.
- Applications include genetically modified crops for pest resistance and enhanced nutrition (e.g., Bt crops, Golden Rice).
Answer: Recombinant DNA (rDNA) technology, often referred to as genetic engineering, is a powerful molecular biology technique that allows for the deliberate modification of an organism's genetic material. The fundamental principle involves combining genetic material from different sources to create novel DNA sequences, which are then introduced into a host organism for replication and expression. The core principle hinges on the use of specific enzymes. Firstly, restriction enzymes, acting as 'molecul...