Q4. Describe the steps involved in DNA microarray in details.
Key Points:
- DNA microarray measures thousands of gene expression levels simultaneously.
- RNA is isolated, reverse transcribed into cDNA, and labeled with fluorophores.
- Labeled cDNA targets hybridize to complementary probes on a solid array surface.
- Washing removes non-specifically bound molecules, ensuring specific signal detection.
Answer: DNA microarray is a high-throughput technology fundamental to OMICS studies, enabling the simultaneous measurement of the expression levels of thousands of genes. This powerful tool is extensively used for gene expression profiling, comparing transcriptomic changes between different biological samples under varying conditions, such as disease states, drug treatments, or developmental stages. While not an experimental step, understanding the array's nature is crucial. A DNA microarray consists o...